Maternal karyogene and cytoplasmic genotype affect the induction efficiency of doubled haploid inducer in Brassica napus

Abstract Background Artificial synthesis of octoploid rapeseed double haploid (DH) induction lines Y3380 and Y3560 was made possible by interspecific hybridization genome doubling techniques. Production pure DH provides a new way to achieve homozygosity earlier in B.napus . Previously, the mechanism induction, whether has obvious maternal genotypic differences or not, are not known so far. Results In this study, different karyogene cytoplasmic genotype were pollinated with previously reported inducers e.g. Y3560. Our study presents fine comparison genotypes unravel induction. Ploidy identification, fertility SSR marker analysis induced F 1 generation, revealed that ploidy phenotype plants consistent type maternal, rather than paternal parent. The SNP chip efficiency affected when same. However, also karyogenes same, offspring ogura cytoplasm showed high frequency inducer gene low-frequency infiltration. Conclusion effect is influenced interaction between genotype, results from partial progeny chromosomes indicate process may be attributed selective elimination chromosome. This basis for exploring , insights utilization molecular breeding.